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. 2018 Dec 20;9:2966. doi: 10.3389/fimmu.2018.02966

Figure 5.

Figure 5

Effect of Pen (43-56)-NBD on cytokine-induced alterations of (A) COX2, (B) IκBα, and (C) phosphorylated-P65 proteins. (Left) representative Western blots demonstrating protein responses at indicated time points following application of 10 ng/ml IL1β either alone or following pre-incubation for 1 h with 100 μM of Pen(43-56)-NBD peptide prior to IL1β addition. Actin expression displayed beneath as loading control. (Right) Scatter plots demonstrating protein signal raw optical density values comparing IL1β alone or 100 μM Pen-NBD plus IL1β experiments.*Significant difference from raw optical density values between IL1β alone or 100 μM Pen-NBD plus IL1β groups (n = 3–4, one-way ANOVA with Bonferroni's post-hoc correction).