Figure 3.

Effects of HG and different factors on EMT in NRK-52E cells. (A) Morphological changes, (B) E-cadherin protein expression, (C) α-SMA protein expression, (D) E-cadherin mRNA expression and (E) α-SMA mRNA expression in NRK-52E cells. **P<0.01 vs. NG+MA and ##P<0.01 vs. HG. HG, high glucose (60 mmol/l); EMT, epithelial-mesenchymal transition; E-cadherin, epithelial cadherin; SMA, smooth muscle actin; NG, normal glucose (5.56 mmol/l); MA, mannitol; DMSO, dimethylsulfoxide; TGF, transforming growth factor; NAC, N-acetylcysteine; Rap, rapamycin.