Figure 5.

Effects of anti-CRT on the production of ROS and the secretion of type I collagen in SP-A-induced HK-2 cells. HK-2 cells were pre-treated with anti-CRT and then incubated with 10 µg/ml SP-A for 60 min. (A) Anti-CRT treatment attenuated the production of ROS in a time-dependent manner. (B) Anti-CRT treatment relieved the secretion of type I collagen in a time-dependent manner. ***P<0.001 vs. SP-A group. SP-A, surfactant protein A; ROS, reactive oxygen species; CRT, calreticulin; NC, normal control.