Figure 7.

Effects of anti-CRT on the expression of MMP2, MMP9 and TIMP1 protein. HK-2 cells were pre-treated with anti-CRT and then incubated with 10 µg/ml SP-A for 60 min. (A) Western blot bands for MMP2, MMP9 and TIMP1. Relative protein levels of (B) MMP2, (C) MMP9 and (D) TIMP1. GAPDH was used as an internal reference. ^##P<0.01 vs. NC; **P<0.01 vs. SP-A group. SP-A, surfactant protein A; NC, normal control; MMP, matrix metalloproteinase; CRT, calreticulin; TIMP1, tissue inhibitor of metalloproteinase 1.