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. 2018 Nov 13;17(1):165–174. doi: 10.3892/etm.2018.6964

Figure 6.

Figure 6.

Impact of Tyk2/Stat1 signaling pathway activation on the epithelial-mesenchymal transition process of BEAS-2B cells. RNA interference was used to silence Tyk2 expression in CLDN12-expressing cells. (A) Western blot analysis was used to examine the effects of silencing Tyk2, the activation of the Stat1 signaling pathway, E-Cadherin expression and Vimentin expression in the BEAS-2B cell line. (B) Corresponding statistical analysis of the protein expression levels. (C) The Transwell chamber method was used to detect the impact of Tyk2 silencing on the invasive ability of cells in vitro (magnification, ×200). (D) Corresponding statistical analysis of invasive cells. (E) The wound-healing assay was used to detect the migration ability of the BEAS-2B cell line in vitro. Analysis of variance and Dunnett's multiple comparisons test was performed. *P<0.05 and **P<0.01 vs. BEAS-2B CLDN12 group or as indicated. Tyk2, tyrosine kinase 2; Stat1, signal transducer and activator of transcription 1; E-Cadherin, epithelial-Cadherin.