Table I.
Quantitative polymerase chain reaction primer sequences of HMGB1, TLR4, NF-κB, RAGE, TNF-α, IL-6, PPAR, SIRT1 and internal reference 28S.
| Gene | Sequence (5′-3′) |
|---|---|
| HMGB1-F | CTAGCCCTGTCCTGGTGGTATT |
| HMGB1-R | CCAATTTACAACCCCCAGACTGT |
| TLR4-F | TCAACCCCCTGAAGATCTTAAGAA |
| TLR4-R | ATGCCTTGTCTTCAATTGTCTCAA |
| RELA | TGGCTTCTATGAGGCTGAACTCT |
| (NFκB3P65)-F | |
| RELA | GGATCCCCAGGTTCTGGAA |
| (NFκB3P65)-R | |
| RAGE-F | AGGAAAGCCCTCCTGTCAACA |
| RAGE-R | CACAGAGCCTGCAGCTTGTC |
| TNF-α-F | CCACCACGCTCTTCTGTCTA |
| TNF-α-R | ACTGATGAGAGGGAGCCCATT |
| IL-6-F | TCTGGTCTTCTGGAGTTCCGT |
| IL-6-R | GCATTGGAAGTTGGGGTAGGA |
| PPARγ-F | TGACTTGGCCATATTTATAGCTGTCA |
| PPARγ-R | GATGTCCTCGATGGGCTTCA |
| Sirt1-F | GCTGGCCTAATAGACTTGCAAAG |
| Sirt1-R | ATGTAACGATTTGGTGGTACAAACA |
| 28S ribosomal | TGCCATGGTAATCCTGCTCA |
| RNA-F | |
| 28S ribosomal | CCTCAGCCAAGCACATACACC |
| RNA-R |
F, forward; R, reverse; HMGB1, high mobility group box 1 protein; RAGE, receptor for advanced glycosylation end products; TLR, toll-like receptor; TNF-α, tumor necrosis factor α; IL, interleukin; NF-κB, nuclear factor kappa-light-chain-enhancer of active B cells; RELA, nuclear factor NF-κB p65 subunit; PPAR, peroxisome proliferator-activated receptor; SIRT1, NAD-dependent protein deacetylase sirtuin-1.