Table I.

Quantitative polymerase chain reaction primer sequences of HMGB1, TLR4, NF-κB, RAGE, TNF-α, IL-6, PPAR, SIRT1 and internal reference 28S.

Gene	Sequence (5′-3′)
HMGB1-F	CTAGCCCTGTCCTGGTGGTATT
HMGB1-R	CCAATTTACAACCCCCAGACTGT
TLR4-F	TCAACCCCCTGAAGATCTTAAGAA
TLR4-R	ATGCCTTGTCTTCAATTGTCTCAA
RELA	TGGCTTCTATGAGGCTGAACTCT
(NFκB3P65)-F
RELA	GGATCCCCAGGTTCTGGAA
(NFκB3P65)-R
RAGE-F	AGGAAAGCCCTCCTGTCAACA
RAGE-R	CACAGAGCCTGCAGCTTGTC
TNF-α-F	CCACCACGCTCTTCTGTCTA
TNF-α-R	ACTGATGAGAGGGAGCCCATT
IL-6-F	TCTGGTCTTCTGGAGTTCCGT
IL-6-R	GCATTGGAAGTTGGGGTAGGA
PPARγ-F	TGACTTGGCCATATTTATAGCTGTCA
PPARγ-R	GATGTCCTCGATGGGCTTCA
Sirt1-F	GCTGGCCTAATAGACTTGCAAAG
Sirt1-R	ATGTAACGATTTGGTGGTACAAACA
28S ribosomal	TGCCATGGTAATCCTGCTCA
RNA-F
28S ribosomal	CCTCAGCCAAGCACATACACC
RNA-R

F, forward; R, reverse; HMGB1, high mobility group box 1 protein; RAGE, receptor for advanced glycosylation end products; TLR, toll-like receptor; TNF-α, tumor necrosis factor α; IL, interleukin; NF-κB, nuclear factor kappa-light-chain-enhancer of active B cells; RELA, nuclear factor NF-κB p65 subunit; PPAR, peroxisome proliferator-activated receptor; SIRT1, NAD-dependent protein deacetylase sirtuin-1.