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. 2018 Nov 9;17(1):79–90. doi: 10.3892/etm.2018.6947

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Early and late stages of BMSC osteogenic differentiation, as determined by ALP staining and ARS, respectively. (A) Representative images of ALP staining after 14-day culture and ARS after 21-day culture. (B) ALP activity results. (C) Summary of ARS results. The PCL/COL/SIM-PLGA construct significantly promoted the osteogenic differentiation of BMSCs in both the early and late stages. *P<0.05, as indicated. PLGA, poly(lactic-co-glycolic acid); SIM, simvastatin; COL, collagen; PCL, poly(ε-caprolactone); BMSC, bone marrow-derived mesenchymal stem cells; ALP, alkaline phosphatase; ARS, Alizarin red staining; OD, optical density.