Figure 2.

Apoptosis of HT22 cells is assessed by Annexin V-FITC/PI staining. (A) Flow cytometry analysis of HT22 cell viability and apoptosis at 24 h after indicated treatments. The Annexin V-FITC⁺/PI⁻ cells (viable apoptotic cells) and Annexin V-FITC⁺/PI⁺ cells (non-viable apoptotic cells) were considered apoptotic cells and counted. (B) The data are presented as the mean ± standard deviation of the results from three independent experiments. **P<0.01 vs. HT22; ^##P<0.01 vs. Isoflurane; and ^{^^}P<0.01 vs. Bupivacaine. FITC, fluorescein isothiocyanate; PI, propidium iodine; IGF-1, insulin-like growth factor 1; Dex, dexmedetomidine.