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. 2018 Nov 12;17(1):63–70. doi: 10.3892/etm.2018.6956

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Copyright: © Bao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Apoptosis of HT22 cells is assessed by Annexin V-FITC/PI staining. (A) Flow cytometry analysis of HT22 cell viability and apoptosis at 24 h after indicated treatments. The Annexin V-FITC⁺/PI⁻ cells (viable apoptotic cells) and Annexin V-FITC⁺/PI⁺ cells (non-viable apoptotic cells) were considered apoptotic cells and counted. (B) The data are presented as the mean ± standard deviation of the results from three independent experiments. **P<0.01 vs. HT22; ^##P<0.01 vs. Isoflurane; and ^{^^}P<0.01 vs. Bupivacaine. FITC, fluorescein isothiocyanate; PI, propidium iodine; IGF-1, insulin-like growth factor 1; Dex, dexmedetomidine.