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. 2018 Nov 12;17(1):63–70. doi: 10.3892/etm.2018.6956

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Copyright: © Bao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Anesthetics induce cell death primarily through the intrinsic apoptosis pathway. (A) The expression of Bax, Bcl-2 and cyto c were detected by western blot analysis. β-actin was used as an equal loading control. The quantitative analysis of the levels of (B) Bax, (C) Bcl-2 and (D) cyto c by one-way analysis of variance and Dunnett's post-hoc test. n=3; data are presented as mean ± standard deviation; **P<0.01 vs. HT22; ^##P<0.01 Isoflurane + Dex vs. Isoflurane; ^#P<0.05 Isoflurane + IGF 1 vs. Isoflurane; ^{^^}P<0.01 Bupivacaine + Dex vs. Bupivacaine and ^{^}P<0.05 Bupivacaine + IGF 1 vs. Bupivacaine. cyto c, cytochrome c; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; IGF-1, insulin-like growth factor 1; Dex, dexmedetomidine.