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. 2018 Nov 22;23(1):29–38. doi: 10.1111/jcmm.13658

Figure 4.

Figure 4

MiR‐9‐5p regulated expression of QKI‐5. A, The targeting relationship between miR‐9‐5p and QKI‐5. B, The luciferase intensity of DU 145 cells cotransfected with QKI‐5 wild‐type and miR‐9‐5p was reduced. **P < .01, compared with miR‐NC group. C, The QKI‐5 expression level of pcDNA3.1‐QKI‐5 group was higher, whereas miR‐9‐5p mimics group was reduced by qRT‐PCR. *P < .05, **P < .01, compared with NC group, # P < .05, ## P < .01, compared with miR‐9‐5p mimics group, && P < .01, compared with pcDNA3.1‐ QKI‐5 group. D, The cell activity of miR‐9‐5p mimics group increased, whereas pcDNA3.1‐ QKI‐5 group was inhibited by CCK‐8 assay. *P < .05, compared with NC group, # P < .05, compared with miR‐9‐5p mimics group, && P < .01, compared with pcDNA3.1‐MEG3 group. E, F, The cell apoptosis rate of miR‐9‐5p mimics group was decreased, whereas pcDNA3.1‐QKI‐5 group was increased by FCM assay. *P < .05, **P < .01, compared with NC group, # P < .05, ## P < .01, compared with miR‐9‐5p mimics group, && P < .01, compared with pcDNA3.1‐ QKI‐5 group. G, H, Cell invasion ability of miR‐9‐5p mimics group was promoted, whereas pcDNA3.1‐QKI‐5 group was inhibited by transwell assay. *P < .05, compared with NC group, # P < .05, ## P < .01, compared with miR‐9‐5p mimics group, & P < .05, compared with pcDNA3.1‐QKI‐5 group. Scale bar = 20 μm. I, QKI‐5 expression level of miR‐9‐5p mimics group was decreased, whereas pcDNA3.1‐QKI‐5 group was increased detected by Western blot. **P < .01, compared with NC group, ## P < .01, compared with miR‐9‐5p mimics group. && P < .01, compared with pcDNA3.1‐QKI‐5 group