Figure 6.

Mechanism of TES modulation of the calcineurin‐NFAT Axis. A, The calcineurin activity of NRVMs that were transduced with the indicated plasmids and stimulated by AngII for 30 min was evaluated. B, Immunofluorescence staining and quantitative analysis of the nuclear translocation of NFAT (n = 100 cells). C, Real‐time PCR analysis of the 1.4 isoform of the calcineurin‐interacting protein (MCIP 1.4) in mouse hearts from the indicated experimental groups (n = 6). D, A GST pull‐down assay was performed to examine the direct interaction between calcineurin and TES. E, Co‐IP assays proving the existence of an interaction between calcineurin and TES in vitro. F, Colocalization of TES and calcineurin in the cytoplasm. G, Co‐IP assays proving the existence of an interaction between calcineurin and TES in vivo. H, Co‐IP assays proving the existence of an interaction between calcineurin and TES in H9c2 cells stimulated with AngII. I, Relative luciferase activity of the NFAT promoter in NRVMs cotransduction with adeno‐associated viruses