Fig 7. Mapping of the DNA region containing the cis-regulatory elements of uvrY recognized by NhaR.
PCR- amplified fragments from pJEK1292 (A) and p1263 (B) were incubated with increasing amounts of NhaR protein for 20 min. at room temperature in a buffer containing 10mM Tris pH7.5, 50mM KCl, 1mM DTT, 5mM MgCl2 and 2.5% glycerol and detected by immunoblotting. In “B” lower concentrations of NhaR were removed and lines 2–6 were moved left next to the control line 1. Boxed lines–controls without NhaR.