Figure 2.

S100A10 is succinylated at lysine 47 in GC. A, Immunoprecipitation analysis of S100A10 succinylation in GC tissues. B, Identification of succinylated S100A10 peptides by mass spectrometry of GC tissues. C, Protein amino acid sequence alignment of S100A10 surrounding K47 from various organisms. K47 is shown in red. The peptide sequence used for preparing the antibody is indicated. D, K47 is the primary succinylation site of S100A10. The indicated plasmids were transfected into MGC‐803 cells and proteins were immunoprecipitated, followed by western blotting for succinylation analysis. E, Western blot analysis of S100A10 K47_succ expression in various cell lines. F, Western blot analysis of the relative levels of S100A10 K47_succ expression in seven pairs of GC and adjacent non‐cancerous tissues. G, Immunohistochemistry of S100A10 K47_succ in adjacent normal tissues, GC tissues, normal lymph nodes and metastatic lymph nodes of GC patients. Results are derived from ten tissue slides. Data are presented as mean ± SEM; *P < 0.05