Figure 6.

Succinylation mimetic S100A10 K47E mutant enhances cancer cell invasion and migration. A and B, Overexpression of WT, K47R, or K47E mutants were determined by western blotting in MGC‐803 (A) and AGS cells (B). C, S100A10 K47R or K47E mutant promotes cell migration. The migration of transfected MGC‐803 cells was analysed by wound‐healing assay. Scale bars represent 100 μm. D, The effect of S100A10 WT, K47R or K47E on AGS cell invasion was determined. E, Overexpression of WT or K47R/E mutants in B16F10 cells was determined by western blotting. F, Mouse model of B6F10 lung metastasis, visible as black dots on the surface of lungs from three groups. G, Representative H&E sections of lung tissues from each group. H, Total numbers of melanoma lung metastatic colonies observed in these groups. Data shown are representative of three independent experiments. Scale bars represent 100 μm. Date are presented as mean ± SEM; **P < 0.01, ***P < 0.001