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TDD‐mediated regulation of HMBOX1 expression in EA.hy926 cells. (A) HMBOX1 mRNA expression was analyzed by FQ‐PCR and normalized mRNA values relative to GAPDH levels were plotted. (B) Effect of TDD on HMBOX1 protein levels was evaluated by western blot with analysis of β‐actin expression as loading control. The data show the mean ± S.D. of three independent experiments. *P < 0.05 and **P < 0.01, compared to the TDD (‐) group.
