Figure 4.

SPOP promotes GLI2/3 ubiquitination and degradation. (A, B) Fg‐SPOP could pull down Myc‐GLI2 (A) and Myc‐GLI3 (B) in A549 cells. (C) Endogenous SPOP interacted with endogenous GLI2 and GLI3 in A549 cells. (D) SPOP promoted GLI2/3 degradation in A549 cells, which was abolished by MG132 treatment. (E) WB analysis of WT and SPOP KO A549 cells. (F) SPOP bound GLI2/3 through its N‐terminal MATH domain. (G) WB assay of A549 cells transfected with WT SPOP or different SPOP mutants. (H) Ubiquitination assay of A549 cells transfected with indicated plasmids