Figure 9.

Dll1‐dependent Notch1 signaling contributes to cisplatin‐induced renal tubular cell death. A and B, Western blot images and bar graphs (n = 3) confirming Dll1 knockdown in Dll1 siRNA‐transfected HK‐2 cells. C, bar graphs summarizing the levels of cleaved Notch1 in scrambled (control; Scrm) siRNA‐ and Dll1 siRNA‐transfected HK‐2 cells (n = 6 each). D, kinetic curves (n = 12 each) demonstrating that cisplatin (30 μmol L⁻¹)‐induced caspase‐3/7 activity in HK‐2 is diminished in Dll1 siRNA‐transfected HK‐2 cells. ^$ P < 0.05 vs Scrm siRNA; ^# P < 0.05 vs Scrm siRNA + cisplatin (8‐22 h for “D”); *P < 0.05 vs Scrm and Dll1 siRNA + Captisol (8‐22 h)