Table 1.

Oligonucleotides used in this study

Gene	Forward primer	Reverse primer	Tan	Cycles
ARID1A	TCTTGCCCATCTGATCCATT	CCAACAAAGGAGCCACCAC	60°C	40
ATF3	AAGAACGAGAAGCAGCATTTGAT	TTCTGAGCCCGGACAATACAC	60°C	40
CDKN1A	CCTCATCCCGTGTTCTCCTTT	GTACCACCCAGCGGACAAGT	60°C	40
DHRS2	CTCCATGTAGGGCAGCAACT	GTAGGGAGCACTCTGGGGAC	60°C	40
DHRS2 genotyp. primer pair 1	GGAAGGACAGTGGAGAGAGG	CCGACTGTATTTCTGTGCCC	60°C	35
DHRS2 genotyp. Primer pair 2	AGAGCTGGGTAGAGGAAGGA	TACAGGCACAGGTCACCAAA	55°C	35
DUSP1	GTACATCAAGTCCATCTGAC	GGTTCTTCTAGGAGTAGACA	60°C	40
FGF13	TGAATTTGCACTCAGGTGTGA	GTCTGCGAGTGGTGGCTATC	60°C	40
FOS	GAGAGCTGGTAGTTAGTAGCATGTTGA	AATTCCAATAATGAACCCAATAGATTAGTTA	60°C	40
GADD45B	GTCGGCCAAGTTGATGAAT	CACGATGTTGATGTCGTTGT	60°C	40
GAPDH	TGCCAAATATGATGACATCAAGAA	GGAGTGGGTGTCGCTGTTG	60°C	40
ID2	TCAGCCTGCATCACCAGAGA	CTGCAAGGACAGGATGCTGATA	60°C	40
MYC	CGTCTCCACACATCAGCACAA	CACTGTCCAACTTGACCCTCTTG	60°C	40
P53	TTGCAATAGGTGTGCGTCAGA	AGTGCAGGCCAACTTGTTCAG	60°C	40
RHOB	GGGACAGAAGTGCTTCACCT	CGACGTCATTCTCATGTGCT	60°C	40
TUFT1	CCTGTCAGTTCACCCTGGAG	AACTGGTGTACCCTGGTGGA	60°C	40
VAMP1	CAGTCCCTTCTGTCCCTTCA	CAGCCTCCGGAGAGGAA	60°C	40
ZMYND11	TTGTTAAACGTGCCATGACC	GCATGTGTGGAGACAGAGGA	60°C	40

Gene	gRNA sequence
DHRS2 gRNA 1	5′‐CTTTGCCATCGCCCGACGTC‐3′
DHRS2 gRNA 2	5′‐GCCATACCTGTTCTCCATGT‐3′
DHRS2 gRNA 3	5′‐CCCAGAGTGCTCCCTACCAG‐3′