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. 2018 Oct 15;23(1):439–452. doi: 10.1111/jcmm.13947

Figure 5.

Figure 5

Knockdown of lncRNA‐MALAT1 interferes with hypoxia‐induced autophagy in human endometrial stromal cells. A, qRT‐PCR was used to examine the knockdown efficiency of lncRNA‐MALAT1 siRNA. (B‐C) Representative Western blots of Beclin1 and LC3 protein in human endometrial stromal cells transfected with negative control siRNA or lncRNA‐MALAT1 specific siRNA in the presence or absence of hypoxia for 24 h. D, Representative immunofluorescence image of GFP‐LC3 puncta in human endometrial stromal cells transfected with negative control siRNA or lncRNA‐MALAT1 specific siRNA under hypoxia condition for 24 h. Photographs were taken at magnifications of 1600×. E, Representative images of human endometrial stromal cells transfected with negative control siRNA or lncRNA‐MALAT1 specific siRNA under hypoxia condition for 24 h, and then were analysed by Transmission electron microscopy (TEM). Autophagical vacuoles containing organelle remnants were highlighted by red arrows. The protein expression levels were quantified by Image J software and normalized to GAPDH protein levels. The data are presented as the means ± SD of three independent experiments (*P < 0.05; **P < 0.01)