Fig 6. Palmitoylation inhibition induces changes in the subcellular localization of HEV ORF3 protein.
S10-3 cells were transfected with either pCMVORF3-GFP (ORF31-113) or pCMVORF31-28-GFP (ORF31-28). One d post-transfection, cells were treated for 24 h with 25 μM 2-bromopalmitate (2-BP) and analyzed by confocal laser scanning microscopy after staining of the nuclei by DAPI. Scale bars represent 10 μm.