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. 2018 Dec 7;14(12):e1007840. doi: 10.1371/journal.pgen.1007840

Fig 7. VANG-1/Vangl controls Dvl localization and phosphorylation.

Fig 7

(A) Expression of endogenously tagged mNG::MIG-5/Dvl [41] in the QL neuroblast (outlined with dotted line). Punctae of mNG::MIG-5 at the cell membrane are indicated with yellow arrows. wrt-2 promoter directed expression of PH::mCherry and H2B::mCherry was used to mark the Q neuroblasts and seam cells. VANG-1 was overexpressed using the hsp16 heat-shock (HS) promoter (1 hr. heat-shock). Scale bar = 10 μm. (B) Quantification of MIG-5/Dvl punctae on the Q neuroblast plasma membrane. Statistical significance was calculated using a Student’s t-test. **, p<0.01. (C) Loss of Vangl1/2 increases Dvl2 and Dvl3 phosphorylation. Endogenous Dvl2 and Dvl3 were detected using specific antibodies. Phosphorylation of Dvl2 and Dvl3 (indicated by arrows with a p) was quantified in wild type and Vangl1/2 double mutant (DKO) cells. Statistical significance was calculated using a Student’s t-test.*, p<0.05; **, p<0.01.