Figure 6.

Ang II‐induced JNK phosphorylation is reversed by Mst1 deficiency via enhanced binding of Trx to ASK1. A: Trx/ASK1 protein binding in myocardial tissues at 7 weeks after chronic Ang II pump infusion (n = 9). Histogram: The relative intensity of Trx/ASK1 Complex. B: Immunoblots and quantitative analyses of Mst1/GAPDH, p‐JNK/JNK, ASK1/GAPDH, Trx/GAPDH and p47^phox/GAPDH (n = 9). Histogram: Relative intensity of Mst1/GAPDH, p‐JNK/JNK, ASK1/GAPDH, Trx/GAPDH, and p47^phox/GAPDH. C: Representative images of immunofluorescent double‐labelled co‐localisation of Trx and ASK1 (n = 9). Histogram: The relative ratio of green puncta (Trx) overlaps red puncta (ASK1). *P < 0.05 vs Mst1^fl/fl group; ^† P < 0.05 vs Mst1^Δ/Δ group; ^&ddagger; P < 0.05 vs Ang II+ Mst1^fl/fl group