Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Aqp4 | MGI:107387 | ||
| Gene (Mus musculus) |
Snta1 | NCBI_gene: 20648, MGI:101772 |
||
| Strain, strain background (Mus musculus) |
Aqp4 null/eGFP knock-in mice |
RIKEN BRC | RBRC06500; SJL.Cg-Aqp4<tm1.1(GFP) Mysi>/MysiRbrc; CDB0758K-1 |
|
| Strain, strain background (Mus musculus) |
Aqp4 knockout mice |
PNAS 108, 846–851 (2011). PMID: 21187412 |
OIU | |
| Strain, strain background (Mus musculus) |
C57BL/6N | Beijing Vital River Laboratory Animal Technology Co., Ltd. |
213 | |
| Strain, strain background (Mus musculus, C57Bl/6J) |
male wild type (WT) | Jackson Laboratories, 000664 |
RRID:IMSR_JAX:000664 | |
| Strain, strain background (Mus musculus, C57BL/6NCrl) |
male wild type (WT) | Charles River, 027 |
RRID:IMSR_CRL:27 | |
| Strain, strain background (Mus musculus, C57Bl/6J) |
male Snta1-/- | Jackson Laboratories, 012940, PMID:10995443 |
RRID:MGI:2181419 | |
| Cell line (Mus musculus) |
E14K ES | J Neurosci Res. 2005 Nov 15;82 (4): 458–64. PMID: 16237719 |
129P2/OlaHsd | |
| Transfected construct (Mus musculus) |
AQP4 replacement targeting vector |
J Neurosci Res. 2005 Nov 15;82(4): 458–64. PMID: 16237719 |
Constructed using the positive– negative selection cassettes derived from the vectors pPolII long neo bpA and pXhoMC1TK, containing the neoR and HSVtk genes, respectively. |
|
| Biological sample (Mus musculus) |
CD1 blastocysts | Beijing Vital River Laboratory Animal Technology Co., Ltd. |
201 | |
| Antibody | anti-Aqp4 (rabbit polyclonal) |
Sigma | A5971, RRID:AB_258270 |
(1:1000) |
| Antibody | biotinylated- conjugated goat anti-rabbit IgG (polyclonal) |
Vector Laboratories |
BA-1000, RRID:AB_2313606 |
(1:200) |
| Antibody | AQP4 (rabbit primary Ab) (polyclonal) |
Millipore | AB3594, RRID:AB_91530 |
(1:400–500) |
| Antibody | Alexa Fluor 594 (donkey anti-rabbit secondary Ab) (polyclonal) |
ThermoFisher | A21207, RRID:AB_141637 |
(1:500) |
| Antibody | Cy3-conjugated donkey anti-rabbit (polyclonal) |
Jackson Immuno Research |
711-165-152, RRID:AB_2307443 |
(1:500) |
| Sequence- based reagent |
oligonucleotide primers of Aqp4 and excision sequence |
Transnetyx | Probe ID: Aqp4-2 WT and Aqp4-2 KO |
|
| Sequence- based reagent |
oligonucleotide primers of Aqp4 and Neo |
J Neurosci Res. 2005 Nov 15;82 (4) :458–64. PMID: 16237719 |
AQP4 forward primer, ACC ATA AAC TGG GGT GGC TCA G; WT AQP4 reverse primer, TAG AGG ATG CCG GCT CCA ATG A; and Neo, CAC CGC TGA ATA TGC ATA AGG CA. |
|
| Peptide, recombinant protein |
Beta - Amyloid (1 - 40), HiLyte Fluor 488 - labeled, Human |
Anaspec | AS-60491–01 | 0.5% in aCSF |
| Commercial assay or kit |
Kwik-Cast | World Precision Instruments |
KWIK-CAST | |
| Commercial assay or kit |
Elite ABC Kit | Vector Laboratories |
PK-7200 | |
| Chemical compound, drug |
BDA | Molecular Probes/Thermo Fisher |
D1957; BDA-70,000 | |
| Chemical compound, drug |
Alexa-594-conjugated streptavidin (SA) |
Molecular Probes/Thermo Fisher |
S11227 | (1:1000) |
| Chemical compound, drug |
Alexa Fluor 647 conjugated bovine serum albumin |
Molecular Probes/Thermo Fisher |
A34785 | 0.5–1% in aCSF |
| Chemical compound, drug |
Texas Red conjugated dextran, 3000 MW, paraformaldehyde, chloral hydrate |
Invitrogen, Sigma-Aldrich, Sigma-Aldrich |
D3328, 158127, 47335 U | |
| Software, algorithm |
ImageJ software | https://imagej.nih.gov/ij/index.html | RRID:SCR_003070 | |
| Software, algorithm |
FIJI | RRID:SCR_002285 | ||
| Software, algorithm |
MetaMorph Basic | Molecular Devices |
RRID:SCR_002368 | |
| Software, algorithm |
Matlab | Mathworks | RRID:SCR_001622 | |
| Software, algorithm |
cellSens | Olympus | RRID:SCR_016238 | |
| Other | artificial CSF | Sigma-Aldrich | All componets in ACSF (concentrations in mM): 126.0 NaCl, 3 KCl, 2 MgSO4, 10.0 dextrose, 26.0 NaHCO3, 1.25 NaH2PO4, 2 CaCl2 are purchased from Sigma- Aldrich |