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. 2018 Dec 17;7:e39393. doi: 10.7554/eLife.39393

Figure 3. DJ-1β senses ROS and regulates activity-induced neural plasticity.

(A) DJ-1β is required for ROS and neuronal-activity-induced NMJ elaboration (100 hr ALH). Larvae reared at 25°C. (B) Cell-specific expression of DJ-1βC104A, non-oxidizable on conserved cysteine C104, prevents activity-induced reduction of active zone number. ‘Control’ is aCC/RP2-GAL4 alone. Larvae were reared at 25°C. (C) Activity-generated ROS sensing is dose sensitive. Removal of one copy of DJ-1β (in DJ-1βΔ93 /+ heterozygotes) is sufficient to significantly rescue activity-induced reduction of total dendritic arbor length of motoneurons in 24 hr ALH larvae. ‘Control’ is aCC-FLP-GAL4 alone, heterozygous, achieved by crossing the GAL4 driver to Oregon-R wild type flies.

Figure 3—source data 1. Source data for Figure 3.
elife-39393-fig3-data1.xlsx (39KB, xlsx)
DOI: 10.7554/eLife.39393.010

Figure 3.

Figure 3—figure supplement 1. DJ-1βΔ93 mutant third instar larval NMJs are phenotypically normal.

Figure 3—figure supplement 1.

Representative TEM bouton cross-sectional images showing that presynaptic architecture is intact including active-zones with associated clustered synaptic vesicles (arrowed).
Figure 3—figure supplement 2. DJ-1βΔ93 mutant third instar larval NMJs fail to elaborate bouton number or active zone number upon rearing at 29ºC.

Figure 3—figure supplement 2.

(A) DJ-1β null mutant (DJ-1βΔ93) larvae do not show systemic activity-dependent NMJ elaboration. Normalised bouton number dot plot showing significantly increased NMJ elaboration in OregonR, but not DJ-1βΔ93, larvae reared at 29°C vs 25°C. (B) DJ-1βΔ93 larvae also do not show systemic activity-dependent increase in active zone number. OregonR, but not DJ-1βΔ93, show significantly increased NMJ active zone number when reared at 29°C vs 25°C. Mean ± SEM, ANOVA **p<0.01, ***p<0.001, n = replicate number.
Figure 3—figure supplement 3. 10 mM DEM feeding induces increased bouton number at both muscle four and DA1.

Figure 3—figure supplement 3.

This response is absent in DJ-1β null mutant larvae and is re-established via neuronal (aCC and RP2) specific miss-expression of UAS-DJ-1β. UAS-DJ-1β expression alone elevates bouton number, which is exacerbated when combined with DEM feeding. Normalised bouton number dot plot, Control is aCC-FLP-GAL4 alone in heterozygous condition. Mean ± SEM, ANOVA *p<0.05, ****p<0.0001, n = replicate number.