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. 2018 Nov 28;7:e40126. doi: 10.7554/eLife.40126

Table 2. Primers used for cloning and generation of TAPBPR loop mutants.

Primer name Primer sequence 5ʹ−3ʹ
TAPBPRWT- BamHI-for GCGCGGATCCAGCAGCCTCCATGGGCACACAGGAGGGC
TAPBPRWT- NotI-rev GCGCGCGGCCGCTCAGCTGGGCTGGCTTACA
M22-for GTCCTAGACTGTTTCCTGGTGGCGGCCGGTGGGAGCGGTGGAGCTCTCGCCAGCAGTG
M22-rev CACTGCTGGCGAGAGCTCCACCGCTCCCACCGGCCGCCACCAGGAAACAGTCTAGGAC
M29-for GCGGCCGGTGGGAGCGGTGGAGGTGGCAGCGGCGGTG
M29-rev TCCACCGCTCCCACCGGCCGCCACCAGGAAACAGTCTAGGAC
L30G-for GTGGAGCTGGCGCCAGCAGT
L30G-rev ACTGCTGGCGCCAGCTCCAC
ØG30L-for GGTGGAGGTCTGGGCGGCGGTGC
ØG30L-rev GCACCGCCGCCCAGACCTCCACC