Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) |
HEK293T | American Type Culture Collection |
Cat#: CRL-3216 RRID: CVCL_0063 |
|
| Transfected construct (synthesize) |
pIRES2_EGFP | Addgene | Cat. #: 6029–1 | |
| Transfected construct (synthesize) |
pICherryNeo | Addgene | Cat. #: 52119 | |
| Transfected construct (synthesize) |
Grie1 in pICherryNeo |
Invitrogen GeneArt Gene Synthesis |
||
| Transfected construct (synthesize) |
Grie7 in pIRES2_EGFP |
Invitrogen GeneArt Gene Synthesis |
||
| Biological sample (Branchiostoma lanceolatum) |
whole animal | Collected in the bay of Argelès- sur-Mer, France (latitude 42° 32' 53’ N and longitude 3° 03' 27’ E) |
||
| Biological sample (Branchiostoma lanceolatum) |
nerve chord | Collected in the bay of Argelès-sur- Mer, France (latitude 42° 32' 53’ N and longitude 3° 03' 27’ E) |
||
| Antibody | Mouse anti-HA |
Covance | Cat. #: MMS-101P RRID: AB_291259 |
IF (1:200), WB (1:1000) |
| Antibody | Rabbit anti-c-Myc |
Cell Signalling |
Cat. #: 2272S RRID: AB_10692100 |
IF (1:100), WB (1:1000) |
| Antibody | Mouse anti-GluA2 |
Millipore | Cat. #: MAB397 RRID: AB_2113875 |
IF (1:200), WB (1:1000) |
| Antibody | Alexa Fluor 555 donkey anti- mouse IgG |
Invitrogen | Cat. #: A-31570 RRID: AB_2536180 |
IF (1:1000) |
| Antibody | Alexa Fluor 647 goat anti -rabbit IgG |
Life Technologies | Cat. #: A-21245 RRID: AB_2535813 |
IF (1:500) |
| Antibody | Donkey anti-mouse |
Li-cor | Cat. #: 926–32212 RRID: AB_621847 |
WB (1:7500) |
| Antibody | Donkey anti-rabbit |
Li-cor | Cat. #: 926–68073 RRID: AB_10954442 |
WB (1:7500) |
| Recombinant DNA reagent |
||||
| Sequence- based reagent |
Grie1 gene from B. Lanceolatum |
|||
| Sequence- based reagent |
Grie7
gene from B. Belcheri |
|||
| Sequence-based reagent |
Seqeucne corresponding with rat Gria2 signal peptide |
|||
| Chemical compound, drug |
N-dodecyl-α- maltopyranoside; DDM |
Anatrace | Cat. #: D310HA |
2% w/v |
| Software, algorithm |
pClamp10 | Molecular Devices |
||
| Software, algorithm |
IgorPro | Wavemetrics | ||
| Software, algorithm |
Neuromatic | doi: 10.3389/ fninf.2018. 00014 |
RRID: SCR_004186 | |
| Software, algorithm |
MrBayes 3.2.6 | doi: 10.1093/sysbio/sys029 | ||
| Software, algorithm |
IQTree | doi: 10.1093/molbev/msu300 | ||
| Software, algorithm |
MolProbity | doi: 10.1107/S0907444909042073 | RRID: SCR_014226 |
|
| Software, algorithm |
MIFit | GitHub (Smith, 2010) |
||
| Software, algorithm |
FIJI | doi: 10.1038/nmeth.2019 | RRID: SCR_002285 |
|
| Other | CIPRES Science Gateway |
doi: 10.1109/GCE.2010.5676129 | RRID: SCR_008439 |
Free on-line super computing resource for evolutionary research |