Fig. 2.

Diapause eggs have increased lipid abundance, primarily in the form of diacylglycerides and triacylglycerides. (A) log₁₀ P-value of t-test for differences in lipid abundance between biological replicates of diapause (n=4) and non-diapause (n=4) eggs. Lipids are sorted and color-coded by class; the number of measured lipids within each class is identified parenthetically. The dashed line indicates a false discovery rate (FDR)-corrected P-value of 0.05; the solid line indicates the top 50 lipids by P-value. (B) The relative abundance for each of the top 50 lipids according to P-value in A displayed as a heat map. Each row in the heat map has been normalized such that the color corresponds to the Z-score for that lipid. Red cells represent higher relative abundance (Z-score) in diapause eggs and blue cells represent lower relative abundance in diapause eggs. Colored blocks to the right of the heat map match the lipid class colors used in A. Lipid abbreviations are as follows: CE, ceramide; CL, cardiolipin; DG, diacylglyceride; FFA, free fatty acid; lysoPC, lyso-phosphatidylcholine; lysoPE, lyso-phosphatidylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; Plasm-PE plasmenyl-phosphoethanolamine; PS, phosphatidylserine; SM, sphingomyelin; TG, triacylglyceride.