Fig. 3.

Partial ADAR2 knockdown mimics TTX-evoked KAR upscaling and also occludes any further TTX-mediated upscaling. (A) Representative western blot of total and surface levels of GluK2, EGFR and GAPDH in cells infected with scrambled or partial shRNAs for ADAR2 knockdown (KD) in the presence or absence of TTX. EGFR was used as a negative control, while GAPDH was used as a control to show that only surface proteins were labelled with biotin. (B) Quantification results from A showing surface levels of GluK2 from 10 independent experiments. Surface levels were normalised to their total levels. *P<0.05, **P<0.01, ***P<0.001 (two-way ANOVA with Tukey's multiple comparisons test). (C) Quantification of results from A showing surface levels of EGFR from 10 independent experiments. Surface levels were normalised to their total levels. ns, not significant (P>0.05; two-way ANOVA with Tukey's multiple comparisons test). (D) RT-PCR and BbvI digestion analysis of GluK2 Q/R editing from hippocampal neurons infected with either scrambled or partial shRNAs for ADAR2 KD with or without TTX. (E) Quantification of results from D for five independent experiments. **P<0.01, ***P<0.001 (one-way ANOVA with Tukey's multiple comparisons test).