Figure 5. p110δ kinase inactivation resists leukemia through a T cell–dependent mechanism.

(A and B) CD44⁺CD4⁺ (A) and CD44⁺CD8⁺ (B) T cells were examined in mice from Figure 3, D–F. Bars represent mean ± SD. Arrow denotes adoptive transfer of leukemia cells. A repeated-measures model was applied to the log-transformed data. For the first rechallenge, comparisons were made of day 7 and day 14 after rechallenge versus before rechallenge. For the second rechallenge, comparisons were made of day 6 and day 20 after rechallenge versus before rechallenge. (C) Eμ-TCL1 leukemia cells were adoptively transferred into p110δ^WT/WT (n = 5) and p110δ^D910A/D910A mice. p110δ^D910A/D910A mice were subjected to CD4-depleting (n = 9) or CD8-depleting (n = 9) antibody injections once every 3 days. Isotype controls (CD4 isotype n = 5, CD8 isotype n = 5) were included as controls. Group differences were estimated using mixed-effects models. Data represent mean ± SD. (D) Day 37 after the engraftment, p110δ^D910A/D910A mice in CD4 depletion, CD4 isotype, CD8 depletion, and CD8 isotype groups were euthanized. Spleen, bone marrow, and blood were analyzed for CD5⁺CD19⁺ leukemia cells. Bars represent mean ± SD. (Differences between antibody and corresponding isotype control groups were estimated using ANOVA based on the log-transformed data.)