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. 2018 Dec 3;129(1):230–245. doi: 10.1172/JCI123176

Figure 2. Expression of Ala92-D2 causes ER stress, triggers UPR response.

Figure 2

UPR markers in Thr92-D2HY–expressing (Thr) or Ala92-D2HY–expressing (Ala) cells. (A) Western blot of pIRE1α. (B) Quantification of pIRE1α shown in A. (C) sXBP1 mRNA levels. RQ, relative quantification. (D) Western blot of uncleaved (uc) and cleaved (c) ATF6. (E) Quantification of cATF6 shown in D. (F) CHOP mRNA levels. (G) Western blot of BIP. (H) Quantification of BIP shown in G. (I) BIP mRNA levels. (J) Western blot of pPERK. (K) Quantification of pPERK shown in J. (L) Western blot of pEIF2α. (M) Quantification of pEIF2α shown in L. (N) ATF4 mRNA levels. (O) Western blot of ERGIC53. (P) Quantification of ERGIC53 shown in O. (Q) ERGIC53 mRNA levels. (R–W) Immunofluorescence of Thr92-D2HY or Ala92-D2HY stably expressing cells using the indicated antibodies. Original magnification, APO ×60/1.40 oil objective. Values are shown in a box-and-whiskers plot indicating median and quartiles. n = 4–5/group. Statistical analysis used was the Mann-Whitney U test or Kruskall-Wallis test followed by the Dunn’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.01.