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. 2018 Dec 3;129(1):296–309. doi: 10.1172/JCI123284

Figure 7. ISRIB reverses the effect of p-Eif2α on translation.

Figure 7

(A) Ribo-Seq analysis of kidney extracts from mice treated with LPS for 16 hours with or without ISRIB. Smear plot in which top 10 differentially translated genes are highlighted in red (edgeR exactTest with BH adjusted P values < 0.05, n = 3 per condition). The x axis denote mean log counts per million (logCPM), and the y axis denotes log fold change (logFC). ISRIB was administered 1 hour after LPS. (B and C) Comparative translatome count values mapped to the TCA cycle and peroxisome pathways. (D) S1 proximal tubules from kidneys of control or septic mice were laser microdissected. Original magnification, ×60. Glom, glomerulus. (E) S2/S3 proximal tubules from indicated conditions were manually microdissected. Original magnification, ×5. (F and G) Transcriptomics analysis was performed on S1 and S2/S3 segments (n = 4 for each condition). Select gene expression levels are shown. P values were determined by Student’s t test (2 sided, nonpaired). The boxplot middle line shows the median, the box edges show the 25th and 75th percentiles, and the whiskers show the ×1.5 interquartile range.