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. 2018 Dec 3;129(1):252–267. doi: 10.1172/JCI122064

Figure 5. AMPK phosphorylates ALDH2 and promotes ALDH2 translocation in the absence of LDLR or ALDH2 rs671 mutant.

Figure 5

(A) AMPK activation promotes ALDH2 nuclear translocation in LDLR-KO BMDMs by cellular fractionation (LKO; n = 3). (B) ALDH2 rs671 mutant increases the translocation of ALDH2 in 293T cells (n = 3). (C) AMPK activation leads to ALDH2 nuclear translocation, whereas inhibition of AMPK blocks nuclear translocation of ALDH2. Scale bars: 5 μm. (D) Quantification of ALDH2 in the nucleus (n = 5). (E) LDLR blocks the translocation of ALDH2 even AMPK activation. Scale bars: 5 μm. Quantification shown in F (n = 5). (G) AMPK activation leads to a dose-dependent increase of ALDH2 phosphorylation in the absence of LDLR in LKO macrophages by SuperSep Phos-tag SDS-PAGE. Statistical comparisons were made using ANOVA. All data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.