Figure 3.

Nrf2 activity in allo-T cells contributes to hepatic, intestinal, and thymic GVHD. Lethally irradiated BALB/c recipients received B6 WT TCD BM and 0.5 × 10⁶ B6 WT or Nrf2^−/− T cells. The GVHD target organs of the recipients were analyzed on day 14 after HCT. Representative hematoxylin and eosin (H&E)-stained images of liver, small intestine, and large intestine (A) that were scored for histopathologic damage (B). (C) H&E-stained slides of skin were assessed for the number of apoptotic keratinocytes per centimeter of epidermis. Data represent the mean ± standard error of the mean combined from 2 independent experiments (n = 16 per group). Flow cytometric analysis of thymic damage; representative dot plots show the percentage (D) and number (E) of total CD4⁺CD8⁺ double-positive thymocytes. Data represent the mean + standard deviation combined from 3 independent experiments (n = 18 per group). *P < .05, **P < .01, ***P < .005.