Figure 3.

Integrin-mediated functions are impaired in Tln1^3mutPMNs. (A) Static adhesion of WT and Tln1^3mut PMNs on fibronectin, ICAM-1, and VCAM-1 upon stimulation with TNF-α or PMA compared with resting PMNs (n = 12 mice). (B) Analysis of Talin1, RIAM, Kindlin-3, and Rap1 expression in control and Tln1^3mut PMNs by western blotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading control. (C) Adhesion strengthening of TNF-α–stimulated WT and Tln1^3mut PMNs on ICAM-1 under increasing shear stress (0.5-9.0 dyn/cm²) measured as the number of adherent cells relative to initially adherent cells (n = 16 channels analyzed in 4 independent experiments). (D) Representative fluorescence-activated cell sorter blots illustrating surface levels of integrin β1, β2, and β3 on Gr-1⁺ neutrophils isolated from bone marrow. (E) Relative amount of fluorescently labeled Escherichia coli particles phagocytosed by WT and Tln1^3mut neutrophils (n = 3 mice). All values are given as means ± 95% confidence intervals. *P < .05, **P < .01, ***P < .001.