Table 2.
Intra-day and inter-day accuracy and precision of HPLC assay for methylphenidate hydrochloride and loxapine succinate.
| Medications | Reference value (μg/mL) | Intra-day (n = 6) |
Inter-day (n = 3) |
||||
|---|---|---|---|---|---|---|---|
| Mean ± SD (μg/mL) | Precision (%) | Accuracy ± SD (%) | Mean ± SD (μg/mL) | Precision (%) | Accuracy ± SD (%) | ||
| Methylphenidate hydrochloride | 50 | 48.59 ± 2.09 | 4.31 | 97.19 ± 3.52 | 49.33 ± 1.76 | 3.57 | 98.66 ± 4.19 |
| 75 | 73.05 ± 2.92 | 4.00 | 97.40 ± 3.57 | 74.11 ± 2.68 | 3.61 | 98.81 ± 3.90 | |
| 100 | 94.56 ± 5.70 | 6.03 | 94.56 ± 4.76 | 97.10 ± 4.76 | 4.90 | 97.10 ± 5.70 | |
| Loxapine succinate | 25 | 24.90 ± 0.20 | 0.81 | 99.59 ± 0.80 | 24.98 ± 0.27 | 1.07 | 99.94 ± 1.06 |
| 50 | 52.29 ± 0.49 | 0.94 | 104.57 ± 0.98 | 50.37 ± 2.04 | 4.05 | 100.74 ± 4.08 | |
| 75 | 74.25 ± 0.86 | 1.16 | 99.90 ± 1.15 | 73.90 ± 0.74 | 1.00 | 98.53 ± 0.99 | |