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. 2018 Oct 4;18:79–87. doi: 10.1016/j.molmet.2018.09.010

Figure 6.

Figure 6

Effect of peripheral MTII administration on feeding and neuronal activation in Mc4r-crePVN-GFP and Mc4r-crePVN-MRAP2 male mice on HFD. (A–D) Representative hypothalamic sections immunostained for cfos (red) in male Mc4r-crePVN-GFP (A and C) and male Mc4r-crePVN-MRAP2 mouse (B and D) following IP injection with vehicle (saline; n = 3 per group; A and B) or MTII (n = 6 per group; C and D). (E) Quantification of cfos expression in the PVN of Mc4r-crePVN-GFP and Mc4r-crePVN-MRAP2 male mice injected with saline or MTII. (F) Graph showing feeding response (food intake over 24 h) after peripheral injection of vehicle (saline; n = 3 per group) or MTII (n = 6 per group) in male Mc4r-crePVN-GFP and Mc4r-crePVN-MRAP2 mice on HFD. 3v = third ventricle; PVN = paraventricular nucleus of the hypothalamus. Bar scale in D (for A, B, C, D) represents 100 μm. All data are presented as mean ± SEM. *P < 0.05, **P < 0.01 compared to Mc4r-crePVN-GFP control mice.