Figure 6.

The effect of H19/miRNA‐106b‐5p/TDRG1 axis on CDDP sensitivity of seminoma in vivo. Male mice were subcutaneously injected with tumor cells to establish xenografts and then intraperitoneally injected with CDDP (6 mg/kg body weight) or vehicle every week for three doses. The mice were humanely sacrificed and their tumors were excised 25 d after inoculation. A, The in vivo effects of H19 and miRNA‐106b‐5p on TDRG1 expression were analyzed by immunoblotting. GAPDH served as a loading control. B, Tumor volumes were measured every third day for consecutive seven times. C, Representative photographs of H&E staining of tumor tissues and IHC staining of Ki‐67 are presented. D, The effects of H19, miRNA‐106b‐5p, and TDRG1 on Ki‐67 expression of TCam‐2/CDDP cells were analyzed by IHC assays. H&E, hematoxylin and eosin; IHC, immunohistochemistry; **P < 0.01, ***P < 0.001