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. 2018 Dec 21;9:2736. doi: 10.3389/fimmu.2018.02736

Figure 5.

Figure 5

Effect of L-carnitine on the functionality of primed antigen-specific CD8+ T-cells. (A) Correlation between pS6 expression after activation for 3 h with plate-bound αCD3 and basal neutral lipid (NL) content (left panel) or fatty acid (FA) uptake (right panel). (B–D) MelA-specific naïve CD8+ T-cells were primed in the absence or presence of L-carnitine or TLR8L. MelA-specific CD8+ T-cells were quantified by flow cytometry after 10 days using cognate PE-conjugated ELA/HLA-A2 tetramers (B). Expression of granzyme B (C) and Tbet (D) in primed MelA-specific CD8+ T-cells was measured by flow cytometry after 10 days. Left panels: representative data are shown as histogram plots. Right panels: percent expression of granzyme B or Tbet is shown. Horizontal lines depict mean values. N = 9. Statistical significance was determined using Spearman's rank correlation (A) or the Wilcoxon signed rank test (B–D). *P < 0.05.