Figure 2.

Fas signaling in BMDCs does not affect CD4⁺ T cell proliferation, apoptosis and activation in vivo. Mice were treated as Figure 1A described. (A) Flow cytometry (left) and proportions (right) of naïve CD4⁺ T cells (CD4⁺TCRβ⁺ CD62L⁺CD44⁻) and effector CD4⁺ T cells (CD4⁺TCRβ⁺CD62L⁻CD44⁺) in lung tissues. (B,C) Flow cytometry (left) and proportion (right) of Ki-67 expression in CD4⁺ T cells of lung tissues (B) and mLN (C). (D,E) Flow cytometry (left) and Median Fluorescence Intensity (MFI) of VAD (right) staining in CD4⁺ T cells of lung tissues (D) and mLN (E). ns, not significant. Data are representative of two independent experiments with 4–6 mice per group (A–E). Student's t-tests (A–E) were performed and data were presented as mean ± SEM.