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. 2018 Dec 21;9:3045. doi: 10.3389/fimmu.2018.03045

Figure 4.

Figure 4

Fas signaling in BMDCs instructs Th2 cell differentiation in vitro. (A–D) Co-culture of wild-type and Fas-deficient BMDCs with naïve OT-II CD4+ T cells (CD4+CD25−CD62L+CD44−) in the presence of OVA323−339 with or without HDM for 48 h (A), 3 days (B,D), 18 h (C), and then CD4+ T cells were harvested for analyses. (A) mRNA expression of Il4, Il13, Gata3, Il9, and Il10 was detected by qPCR and normalized to Hprt. (B) Production of IL-4 and IL-13 was detected by ELISA. (C) Flow cytometry of the activation status of OT-II CD4+ T cells. (D) Flow cytometry of Ki-67 expression in OT-II CD4+ T cells. (E) Naïve OT-II CD4+ T cells were labeled with CFSE and then co-cultured with wild-type or Fas-deficient BMDCs in the presence of OVA323−339 and HDM for 4 days. Flow cytometry of CD4+ T cell proliferation. *P < 0.05, ns, not significant. Data are representative of two independent experiments with duplicate or triplicate wells per group (A,B). Student's t-tests were performed and data were presented as mean ± SEM.