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. 2018 Dec 21;9:2989. doi: 10.3389/fimmu.2018.02989

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Copyright © 2018 Swain, Roe, Sebrell, Sidar, Dankoff, VanAusdol, Smythies, Smith and Bimczok.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal microscopy analysis shows endosomal expression pattern of CD103 in human monocyte-derived and gastric DCs. (A) Confocal images of human MoDCs that were permeabilized and labeled with antibodies to CD103 (Alexa488) and CD11c (Alexa555) Nuclei were labeled with DRAQ5. Arrowheads point to intracellular CD103⁺ vesicles in DCs without surface CD103 expression, arrow indicates a DC with both surface and intracellular CD103 expression. Representative images from one of three similar experiments are shown. Bar: 20 μm. (B) Gastric lamina propria cells were permeabilized and labeled with antibodies to CD103 (Alexa488) and HLA-DR (Alexa555). Image shows a gastric HLA-DR⁺ DC. Bar: 10 μm.