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. 2018 Dec 21;9:2989. doi: 10.3389/fimmu.2018.02989

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Copyright © 2018 Swain, Roe, Sebrell, Sidar, Dankoff, VanAusdol, Smythies, Smith and Bimczok.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Partial co-localization of CD103 with endosomal markers in human MoDCs. MoDCs generated in medium alone or in the presence of retinoic acid (RA, 100 nM) were permeabilized and stained with antibodies to CD103 (green) and the following endosomal markers (red): (a–c) clathrin, (e–g) EEA-1, (i–k) Rab11, and (m–o) Rab7a. Images were obtained by confocal microscopy. Co-localization of CD103 with (d) clathrin, (h) EEA-1, (l) Rab11, and (p) Rab7a was calculated as the Manders' colocalization coefficient (M2) using ImageJ. Mean ± SEM of 10 −17 confocal images obtained from two independent experiments. Bar = 20 μm, ^*P ≤ 0.05; ^**P ≤ 0.01; unpaired Student's T-test.