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. 2018 Dec 21;9:3043. doi: 10.3389/fimmu.2018.03043

Figure 2.

Figure 2

Impaired effector CD8+ T cell responses in the absence of pulmonary CD103+ cDC1s. (A) NP366−374-specific CD8+ T cells in the lungs of uninfected, infected wild type, and Clec9A-DTR mice (day 6, 10, and 15 post infection). (B) Kinetics of total CD8+ T cells and NP366−374-specific CD8+ T cells in the lungs of uninfected, infected wild type, and Clec9A-DTR mice. Absolute numbers are shown. (C) Lung virus load was measured by relative quantification of M1 viral protein in infected wild type and Clec9A-DTR mice on day 10 post infection. (D–F) Lung cells were harvested from uninfected, infected wild type, and Clec9A-DTR mice on day 10 post infection and stimulated with PMA/Ionomycin for 3 h followed by Brefeldin A incubation for an additional 3 h. Intracellular IFN-γ and IL-10 staining profiles (d) of pulmonary CD8+ T cells, frequency (E) and total numbers (F) of IFN-γ-producing, IL-10-producing, and IFN-γ/IL-10 double-producing CD8+ T cells in the lungs. (G) IFN-γ and IL-10 BAL levels as measured by sandwich ELISA (H) Relative quantification of IFN-γ and IL-10 transcripts. Data are shown as mean ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Data represent two (B–F) (n = 3–5) independent experiments.