Figure 1.
Inhibition of peripheral blood mononuclear cell (PBMC) proliferation by mesenchymal stem cells (MSCs). Carboxyfluorescein succinimidyl ester (CFSE) labeled 1 × 105 PBMCs were activated with 2 μg/ml PHA and incubated for 4 days with and without MSCs at a ratio of 1:20, 1:10, and 1:5. (A) A representative FACS analysis of CFSE-labeled PBMCs, activated and non-activated, with and without MSCs at different ratios. (B) A graphic presentation of the percent suppression of activated PBMCs with different MSCs at a ratio of 1:20, 1:10 (p < 0.003), and 1:5 (p < 0.001). Data are expressed as the mean of tested samples. Activated condition counts were set to represent 100%.