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. 2018 Dec 21;9:3174. doi: 10.3389/fmicb.2018.03174

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Copyright © 2018 Gan, Yang, Hu, Chen, Zhou, Li, Xu, Wang, Chen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Poly I:C-induced inflammation and NETs in mice with ALI. (A) Infiltration of inflammatory cells and interstitial edema in the lung lobes from poly I:C-stimulated mice, bar = 100 μm; (B-1) cells were counted in the BALF, ^∗p < 0.05; (B-2) protein concentration in the BALF, ^∗p < 0.05; (B-3) neutrophil elastase (NE) activity was evaluated in the BALF, ^∗p < 0.05; (C) flow cytometry analysis of the BALF cells, ^∗p < 0.05; (D) inflammatory cytokines IL-1β, IL-6, and TNF-α in the lung were quantified using qRT-PCR, ^∗p < 0.05; (E) NETs were observed and quantified in lung tissues from mice administered poly I:C. (F) Claudin-5 and p38 MAPK were measured in lung tissues using Western-blot.