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. 2018 Dec 27;8:18086. doi: 10.1038/s41598-018-35923-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of standard and amplified DENV NS1 MagPlex assay in 50% serum. Dilutions of each DENV NS1 variant was prepared in 50% serum and then tested either using the standard (top panels, linear plot left and log plot right) or amplified MagPlex assay (bottom panels, linear plot left and log plot right) using DD7-GS3K as the capture sdAb immobilized on the MagPlex beads and Bt-DD5-GS3K as the tracer.