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. 2018 Dec 27;8:18086. doi: 10.1038/s41598-018-35923-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MagPlex detection of DENV NS1 versus NS1 from other flaviviruses. Dilutions of NS1 were prepared in 50% serum and evaluated using the amplified MagPlex assay with DD7-GS3K as the capture and Bt-DD5-GS3K as the tracer sdAb. The top panels (linear plot left and log plot right) show detection of NS1 from all four DENV serotypes versus NS1 from WNV. While the bottom panels (linear plot left and log plot right) show the comparison of signal produced by NS1 from other flaviviruses versus the signal produced by DENV-4 NS1. The blue line in the right panels represents a signal to background ratio of 2; a value greater or equal to 2 was defined as a positive.