Table 1.
Melting Point (Tm) and dissociation constants (KD) of the sdAb.
| SdAb | Tm °C | KD (nM) | |||
|---|---|---|---|---|---|
| DENV-1 | DENV-2 | DENV-3 | DENV-4 | ||
| DH12 | 68 | 14.4 | 9.8 | 12.4 | 20.9 |
| DD5 | 65 | 2.8 | 4.5 | 3.2 | 24.0 |
| DC4 | 64 | 62.0 | 42.1 | 31.6 | 43.0 |
| DD7 | 59 | 5.4 | 20.3 | 7.0 | 2.4 |
| DD6 | 53 | 7.6 | 2.0 | 1.1 | 2.5 |
| DC7 | 52 | 9.1 | 2.6 | 1.2 | 2.2 |
| DB5 | 65 | NBO | NBO | 4.4 | NBO |
| DH4 | 55 | 10.3 | 8.5 | 23.8 | 8.5 |
| DD1 | 61 | 28.5 | 15.0 | 85.8 | 7.8 |
| DD9 | 59 | 18.0 | 6.7 | 21.8 | 34.8 |
The Tm was determined via the fluorescent dye melt assay and the KD for each sdAb determined from the binding association and dissociation to immobilized NS1 of each variant. NBO denotes that no binding was observed.